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Principle
- Loeffler medium is formulated by Loeffler (1887) and further modified by Perry and Petran (1939). The medium is used as an enrichment medium for primary and secondary isolation and cultivation of fastidious pathogenic microorganisms.
- The medium is composed of special peptone, meat extract, sodium chloride, and dextrose. In medium special peptone, meat extract provides essential growth nutrients.
- Dextrose is the source of fermentable carbohydrates and energy. Sodium chloride maintains cell integrity. For proteolytic activity, the medium is fortified with horse serum.
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Use: Recommended for the cultivation of Corynebacterium diphtheriae from clinical specimens, and also used for the detection of chromogenesis, proteolysis, and the production of ascospores.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light beigecolored powder.
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Prepared medium
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Basal: Light amber colored clear to slightly opalescent solution. After addition of horse serum: Off-white colored opaque solution
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pH
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7.3 ±0.2 at 25 ˚C (8.75 g/ 250 ml + 750 ml sterile Horse Serum )
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