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Principle
- Letheen broth modified is composed of peptone, tryptone, meat extract (the equivalent of beef extract), yeast extract, sodium chloride, sodium bisulfite, lecithin, and polysorbate 80.
- Peptone, tryptone, meat extract, and yeast extract provide nitrogenous and carbonaceous compounds, long-chain amino acids, vitamins, and trace elements for the growth of microorganisms.
- Sodium chloride maintains the equilibrium of the medium. Sodium bisulfite neutralizes the preservative and enhances the growth of microorganisms.
- The lecithin and tween 80 aid the recovery of bacteria from materials containing residues of disinfectant or preservatives used in cosmetics. T
- ween 80 and lecithin act as surface active disinfectant neutralizing agents and nullify other additives in commercial products. Lecithin also neutralizes quaternary ammonium compounds present in cosmetics.
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