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Principle
- Lactose medium is composed of peptone, meat extract (equivalent to beef extract), and lactose. Peptone and meat extract provide nutrients such as amino acids, vitamins, and other trace factors which are essential for the growth of fastidious microorganisms.
- Lactose serves as a carbon source and plays a crucial role in the selection of lactose-fermenting microbes. It is also used as a pre-enrichment, non-selective medium used for the repair of damaged Salmonella cells in processed food and dairy samples.
- Most of the non-Salmonella bacteria ferment lactose while Salmonella does not. As lactose-fermenting bacteria metabolize lactose and the pH of the medium decreases, creating a bacteriostatic effect on competing microorganisms.
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Use: Recommended for selective isolation and differentiation of Lactose fermenting bacteria from pharmaceutical samples and water, sewage, etc. Specifically, the coliforms; E coli by using Durham’s tube for detection of gas production.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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Fine, free flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light to amber, clear solution
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pH
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6.9 ±0.2 at 25 ˚C (13.00 g/ l)
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