Lactose Agar w/ Bromthymol Blue

Principle

• Lactose Agar with bormothymol blue is designed by Chapman et al (1937), is used in the detection and isolation of pathogenic Staphylococci generally coagulase positive.
• On this media Staphylococci are differentiated by their ability to grow at a high pH and in the presence of bromothymol blue.
• Media is composed of proteose peptone and meat extract serves as a source of carbon, nitrogen, long chain amino acids, vitamins and other essential nutrients. Lactose is the fermentable carbohydrate and bromothymol blue as an pH indicator.
• Typical colonies appear deep yellow or blue grey. Coliforms may grow but are differentiated by their appearance. 

Use:

Recommended for isolation of pathogenic Staphylococci from clinical sample.