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Principle
- Iron sulphite agar is composed of tryptone, sodium sulphite, Ferric citrate and agar. Tryptone provides nitrogen and other trace nutrients for the growth of bacteria. Sodium sulphite is a selective agent and sulphite source.
- Ferric citrate is a sulphite reduction indicator. The sulphite-reducing bacteria reduce sulphite to sulphide, which reacts with iron salt and forms a black colour complex.
- Since the sulphite-reducing bacteria forms black colour colonies. Agar is the solidifying agent.
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Use: Recommended for detection of anaerobic sulphide spoilage causing microorganisms in food.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber colored opalescent
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pH
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7.1 ±0.2 at 25 ˚C (26.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber colored opalescent gel
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