Hot-Start DNA Polymerase
Hot-Start Taq DNA polymerase is a chemically modified Taq polymerase; which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach 5 kb (simple template) and 0.5 kb/min separately. Hotstart Taq has 5’-3’ polymerase activity; but no 3’-5’ exonuclease activity. The products of Taq Plus have overhung dA at 3’-end. Hotstart Taq DNA polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.
Size |
250U / 500U |
Product Category |
PCR |
Application |
Amplification of difficult DNA fragments (G-C rich); Low copy PCR assay; Generation of PCR products for TA cloning |
Storage/Stability |
-20°C/1 year |
Shipping |
Gel Packs |