HA (Histamine) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microplate provided in this kit has been pre-coated with Histamine (HA) protein. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Histamine (HA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histamine (HA) in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit Components:
1) Pre-coated Microplate [12 strips x 8 wells]
2) 100x Biotinylated Conjugate [120ul]
3) Biotinylated Conjugate Diluent [10ml]
4) 100x Streptavidin-HRP [120ul]
5) HRP Diluent [12ml]
6) TMB Substrate Solution [9ml]
7) 25x Wash Buffer [20ml]
8) Stop reagent [6ml]
9) Lyophilized Standard [2]
10) Standard Diluent Buffer [20ml]
11) Plate Sealers [2]
12) User Manual [1]
| Size |
1 x 96 Well Plate |
| Product Category |
ELISA (RUO) |
| Reactivity |
General |
| Sample Source |
Serum; Plasma; Tissue Homogenates; Cell Lysates; Cell Culture Supernatant; Other Biological Fluids |
| Assay Method |
Competitive Inhibition ELISA |
| Detection Range |
1.57-100ng/mL |
| Sensitivity |
0.51ng/mL |
| Assay Duration |
2h |
| Synonyms |
Histamin |
| Storage/Stability |
-20°C/1 year; 4°C/6 months |
| Shipping |
Gel Packs |
