Principle
- GN Broth is recommended by the Indian Pharmacopoeia (2017) for the selective isolation of Shigella species with subsequent isolation on a selective medium, XLD Agar. The medium contains polypeptide peptone, which provides amino acids and other nitrogenous substances essential for bacterial growth.
- The selectivity is provided by sodium citrate and sodium deoxycholate which inhibit gram-positive and some gram-negative bacteria such as coliforms.
- Phosphates serve as a buffering system. Sodium chloride maintains osmotic equilibrium.
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Use: Recommended for the enrichment of Shigella from pharmaceutical products in accordance with Indian pharmacopoeia 2017.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber colored opalescent solution
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pH
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7.0 ±0.2 at 25 ˚C (34.00 g/ l)
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