Fluid Thioglycollate Broth

Principle

• Fluid thioglycollate broth is used for detecting microorganisms in normally sterile materials. Media support the growth of a wide variety of fastidious microorganisms having a wide range of growth requirements. Media is composed of tryptone, yeast extract, dextrose, sodium chloride, L-cystine, and sodium thioglycolate. Tryptone provides nitrogen, carbon, amino acids, and vitamins.
• Yeast extract is source of essential carbon, vitamin, nitrogen, and amino acids. Dextrose serves as a carbon and energy source and is included in the media for the early and vigorous growth of many organisms.
• Sodium chloride is used to maintain the osmotic balance. L-cystine and sodium thioglycollate allow anaerobic organisms to grow in aerobic conditions.
• Sodium thioglycollate also acts as a reducing agent and neutralizes the toxic effects of mercurial preservatives and peroxides formed in the medium, thereby promoting anaerobiosis, and making the medium suitable to test materials containing heavy metals.

• Use: Recommended for sterility testing of biologicals and for the cultivation of anaerobes, aerobes, and microaerophiles from pharmaceutical and clinical samples.