Extra-chromosomal DNA / Plasmid Research Services
We offer isolation of cloned plasmid from bacterial culture, native wild plasmid DNA from bacterial culture, Restriction mapping of native wild plasmid & sequencing of the native bacterial plasmid.
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Services We Offer (Description)
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Quantity
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Isolation of cloned plasmid from bacterial culture
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Per clone
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Isolation of native wild-type plasmid DNA from bacterial isolate
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Per culture
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Restriction mapping of native wild-type plasmid
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Per plasmid
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Sequencing of Native bacterial plasmid
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Per base
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Here are some common applications of plasmid research:
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Gene Cloning: Plasmids are widely used as vectors in gene cloning experiments. Scientists can insert specific DNA sequences of interest, such as genes or regulatory elements, into plasmids. These modified plasmids can then be introduced into host cells, where they replicate independently and express the inserted genes. This technique enables the production of large amounts of specific DNA or proteins for further analysis.
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Gene Expression Studies: Plasmids are crucial tools for studying gene expression. By incorporating reporter genes, such as the green fluorescent protein (GFP), into plasmids, researchers can monitor the activity of regulatory elements or observe the spatial and temporal expression patterns of genes in living cells or organisms. This allows the investigation of gene function and regulation.
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Protein Production: Plasmids are commonly used for recombinant protein production. Researchers can clone genes encoding specific proteins into plasmids, which are then introduced into host cells for protein expression. This technique is widely employed in biotechnology and pharmaceutical industries for the production of therapeutic proteins, enzymes, and other valuable biomolecules.
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Gene Therapy: Plasmids are promising tools in gene therapy, an approach to treat genetic disorders by introducing therapeutic genes into patients' cells. Modified plasmids containing therapeutic genes can be delivered to target cells to restore or enhance specific cellular functions. Plasmids offer advantages such as ease of manipulation, versatility, and safety compared to other viral vectors commonly used in gene therapy.
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Antibiotic Resistance Studies: Plasmids often carry genes that confer resistance to antibiotics. Research on plasmid-mediated antibiotic resistance is critical for understanding the mechanisms of antibiotic resistance and developing strategies to combat it. Plasmids can transfer resistance genes between bacteria, leading to the spread of antibiotic resistance in clinical and environmental settings. Studying plasmids helps in identifying and characterizing resistance mechanisms and finding ways to tackle antibiotic resistance.
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Genetic Engineering: Plasmids are essential tools in genetic engineering experiments. Researchers can modify plasmids by introducing specific DNA sequences, deleting or mutagenizing existing sequences, or combining different genetic elements. These modified plasmids can be used to study gene function, investigate regulatory networks, or design synthetic biological systems.
These are just a few examples of the applications of plasmid research. Plasmids are versatile tools in molecular biology and have contributed significantly to our understanding of genetics, gene expression, and various biotechnological and medical advancements.
