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Principle
- EE Broth Mossel is prepared according to the formula of Mossel, Visser, and Cornelissen (1963). Enterobacteria enrichment broth Mossel is used for cultivation of most Enterobacteriaceae, thus assuring the detection of Salmonella, Shigella, and other enteric pathogens and lactose-negative organisms.
- This media is also recommended by USP/EP/IP/JP/BP for testing bile tolerating gram-negative bacteria and microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
- The medium is prepared in accordance with the harmonized principles of USP/EP/IP/JP. Medium is consisting of a pancreatic digest of gelatin, dextrose monohydrate, dehydrated Ox bile, potassium dihydrogen phosphate, disodium hydrogen phosphate, and brilliant green.
- The pancreatic digest of gelatin provides nitrogenous compounds, long-chain amino acids, vitamins, and other essential growth nutrients. The dextrose monohydrate serves as a carbon and energy source.
- The dehydrated Ox bile and brilliant green are selective agents, that inhibit gram-positive bacteria. Potassium dihydrogen phosphate and disodium hydrogen phosphate are buffering agents.
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Use: Enterobacteria enrichment broth Mossel is used for cultivation of most Enterobacteriaceae, (Salmonella, Shigella) and other enteric pathogens and lactose-negative organisms. Media is also recommended by USP/EP/IP/JP/BP for testing bile tolerating gram-negative bacteria and microbial limit testing of food products, and pharmaceutical products.
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