Principle
- EC broth is used for the detection of coliforms during bacteriological examination of water, milk, and food samples. Media was described by Hajna and Perry (1943). Media composed of tryptone, lactose, bile salt, dipotassium phosphate, potassium dihydrogen phosphate and sodium chloride.
- Tryptone provides nitrogen and amino acids and trace nutrients required for the growth of bacteria.
- Lactose is a fermentable carbohydrate, allowing only lactose-fermenting bacteria to grow. Bile salts inhibit gram-positive bacteria including fecal streptococci. Dipotassium phosphate and potassium dihydrogen phosphate are buffering agents that resist changes in the pH of the medium. Gas production in a fermentation tube within 24 hours or less is presumptive evidence of the presence of coliform bacteria.
|
Use: Recommended for the selective enumeration of presumptive Escherichia coli by MPN method from water, food, and clinical samples.
|
PARAMETERS
|
STANDARD VALUES
|
PHYSICAL PARAMETERS:
|
Dehydrated Powder
|
Description
|
A fine, free-flowing, hygroscopic powder.
|
Color
|
Beige-colored powder.
|
Solution
|
Solubility
|
Soluble in Distilled / de-ionized water at 50˚ C
|
Color
|
Light amber colored opalescent
|
pH
|
6.9 ±0.2 at 25 ˚C (37.00 g/ l)
|
|