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Principle
- L-Cystine lactose electrolyte deficient medium composed of peptone, tryptone, meat extract, lactose, L-Cystine, and bromothymol blue. Literature suggests that the Proteous species can be controlled by restricting the electrolytes and by replacing the mannitol with lactose and sucrose with L-Cystine and bromothymol blue.
- Peptone, meat extract, and tryptone serve as the source of all essential nutrients such as amino acids, vitamins, and other trace factors. L-Cystine is added as a growth supplement for cystine-dependent coliforms.
- Lactose is included as a carbon source and plays a crucial role in the selection of lactose-fermenting microbes. Brom Thymol Blue is used as a pH indicator. Organisms capable of fermenting lactose will lower the pH of the medium, resulting in a change in the color of the medium from green to yellow. Agar is used as a solidifying agent.
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