Columbia Agar (Harmonized)

 Principle

• The Columbia agar is prepared in accordance with harmonized principles of USP/EP/IP. Columbia is composed of a pancreatic digest of casein, meat peptic digest, heart pancreatic digest, yeast extract, maize starch, sodium chloride, and agar.
• The pancreatic digest of casein, meat peptic digest, heart pancreatic digest, and yeast extract support the growth of fastidious microorganisms.
• The pancreatic digestion of casein, meat peptic digest, and yeast extract provide nitrogen carbon amino acid, and vitamins.
• The heart's pancreatic digest supplies additional nitrogen and amino acids. Maize starch serves as an energy source and also neutralizes toxic metabolites.
• Sodium chloride maintains the osmotic balance of the medium. Agar is a solidifying agent.

 Use:

Recommended for detection of Clostridium sporogenes from pharmaceutical products and raw materials used in pharmaceutical industries in accordance with the microbial limit testing harmonized principles of USP/EP/IP.