Principle
- The Columbia agar is prepared in accordance with harmonized principles of USP/EP/IP. Columbia is composed of a pancreatic digest of casein, meat peptic digest, heart pancreatic digest, yeast extract, maize starch, sodium chloride, and agar.
- The pancreatic digest of casein, meat peptic digest, heart pancreatic digest, and yeast extract support the growth of fastidious microorganisms.
- The pancreatic digestion of casein, meat peptic digest, and yeast extract provide nitrogen carbon amino acid, and vitamins. The heart's pancreatic digest supplies additional nitrogen and amino acids.
- Maize starch serves as an energy source and also neutralizes toxic metabolites. Sodium chloride maintains the osmotic balance of the medium. Agar is a solidifying agent.
Use:
Recommended for detection of Clostridium sporogenes from pharmaceutical products and raw materials used in pharmaceutical industries in accordance with the microbial limit testing harmonized principles of USP/EP/IP
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Beige-colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber-colored clear to slightly opalescent solution
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pH
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7.30 ±0.2 at 25 ˚C (44.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber-colored clear to slightly opalescent gel
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