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Principle
- Clostridial agar is composed of tryptone, soya peptone, dextrose, sodium chloride, sodium thioglycolate, L-Cystine, sodium formaldehyde sulphoxylate, neomycin sulfate, sodium azide, and agar.
- Tryptone and soya peptone provide nutrients and cofactors required for the growth of clostridia. Dextrose is a carbon source. L-Cystine is an amino acid that enhances clostridial growth, Sodium chloride, sodium thioglycolate, L-cystine, and sodium formaldehyde sulphoxylate maintain the oxidation-reduction potential of the medium.
- Neomycin sulfate and sodium azide inhibits the growth of contaminating bacteria like Proteus, Pseudomonas, and Bacillus. Agar is solidifying agent.
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