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Principle
- Chapman stone agar is a selective medium used for the isolation of staphylococci from food and food products. The media is composed of tryptone, yeast extract, gelatin, lactose, mannitol, sodium chloride, dipotassium phosphate and agar.
- Tryptone is a source of nitrogen, vitamins, and minerals.
- Yeast Extract provides carbon and nitrogen and B-complex vitamins which stimulate bacterial growth. Sodium Chloride, in high concentration, inhibits most bacteria other than staphylococci. Lactose and D-Mannitol are carbohydrates. Gelatin is included for testing liquefaction.
- Agar is the solidifying agent.
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