Principle
- Cetrimide agar is described by King et.al (1954) and is prepared in accordance with the harmonized principles of USP/EP/IP. Recommended as a selective medium for the isolation of Pseudomonas aeruginosa in pharmaceutical testing and microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
- Media consists of pancreatic digestion of gelatin, magnesium chloride, dipotassium sulfate, cetrimide, and agar.
- The pancreatic digest of gelatin and peptone provides essential nutrients, vitamins, nitrogenous factors, and growth factors required for the growth of microorganisms.
- The magnesium chloride and potassium sulfate stimulate pyocyanin and fluorescein production. Cetrimide is a selective agent and inhibits most bacteria by acting as a detergent.
- The cetrimide came in contact with bacteria, causing the release of nitrogen and phosphorous from the bacterial cell other than Pseudomonas aeruginosa.
- Glycerol (not provided) is supplemented as a source of carbon. Agar is the solidifying agent.
Use:
Recommended for the isolation of Pseudomonas aeruginosa from pharmaceutical products in accordance with microbial limit testing by harmonized principles of USP/EP/IP.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Beige-colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber-colored opalescent solution with a slight precipitate
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pH
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7.2 ±0.2 at 25 ˚C (45.50 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber-colored opalescent gel with a slight precipitate
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