Bushnell-Haas agar is a modification of Bushnell Hass broth described by Bushnell and Hass, for the enrichment of hydrocarbon-decomposing microorganisms.
The media is composed of magnesium sulfate, calcium chloride, monopotassium phosphate, ammonium phosphate dibasic, potassium nitrate ferric chloride, and agar.
Magnesium sulfate, calcium chloride and ferric chloride provide necessary trace elements for the growth of bacteria. Monopotassium phosphate and ammonium phosphate dibasic are buffering agents.
Potassium nitrate is a nitrogen source. The media lacks a carbon source and can be fortified with hydrocarbons for evaluation of hydrocarbon decomposing ability. Agar is the solidifying agent.
Bushnell-Haas was recommended for the microbiological examination of fuels by the Society for Industrial Microbiology (SIM) Committee on Microbiological Deterioration of Fuels.
Use: Recommended for isolation of hydrocarbon-degrading microorganisms and enumeration of fuels contaminating microorganisms.
PARAMETERS
STANDARD VALUES
PHYSICAL PARAMETERS:
Dehydrated Powder
Description
A fine, free-flowing, hygroscopic powder.
Color
Tan-colored powder.
Solution
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored with white precipitate.
pH
7.0 ±0.2 at 25 ˚C (20.27 g/ l)
Agar Plates
Solidification
40 ˚C
Color
Light amber colored opalescent gel with yellow-orange precipitate
