Principle
- Brucella agar base is composed of peptone, tryptone, yeast extract, dextrose, sodium chloride, sodium bisulfite, and agar. Peptone and tryptone provide nitrogen and amino acids.
- Yeast extract adds essential vitamins. Dextrose is a carbon source; Sodium Bisulfite is a reducing agent and enhances growth.
- Sodium Chloride maintains the osmotic balance. Agar is the solidifying agent. The media can be enriched with blood (5-10%), which provides additional growth factors for fastidious microorganisms and is used to determine hemolytic reactions.
Use:
Recommended for enrichment, isolation, and cultivation of Brucella or Campylobacter species from clinical and non-clinical specimens.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Cream to light brown colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Beige colored opalescent
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pH
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7.0 ±0.2 at 25 ˚C (43.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light yellow to creamy colored opalescent gel
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