Principle
- Brilliant green bile broth (BGB) medium is composed of peptone, which provides an ample amount of carbon, nitrogen, and essential and non-essential amino acids, along with trace minerals.
- The Bile salts in it inhibit the gram-positive microbes. Lactose is a carbohydrate source. Bacteria that ferment lactose are detected by the gas production trapped inside Durham’s tube.
- Brilliant Green inhibits gram-positive bacteria and many gram-negative bacteria other than coliforms. Bile broth base is used as selective microbial media for the enumeration and detection of the enterococcus family members.
Use:
Recommended for detection and confirmation of coliform bacteria in municipal water, wastewater, food, milk, and dairy products.
PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Greenish beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Emerald green, clear solution
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pH
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7.2 ±0.2 at 25 ˚C (40.00 g/ l)
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