Brain Heart Infusion Broth

Principle

• The medium contains protease peptone and infusions from the calf brain and beef heart which serve as sources of carbon, nitrogen, essential growth factors, amino acids, and vitamins.
• Dextrose is used as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
• Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms. It is highly nutritious and can support the luxuriant growth of a wide variety of microorganisms.
• It can be further enriched by the addition of blood or rendered selective by adding different antibiotics. BHI with 0.5% Polysorbate 80 can be used for detecting Mycobacterium avium-intracellulare complex organisms and M. tuberculosis from blood cultures. 

Use:

Recommended for enrichment, propagation, and cultivation of fastidious pathogenic microorganisms from clinical samples like blood.