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Principle
- BiGGY Agar is a modification of the formula described by Nickerson (1947, 1953), developed for the isolation of sulfite-reducing Candida species. Media is composed of yeast extract, glycine, D-glucose, bismuth sulphite indicator, and agar.
- Yeast Extract provides nitrogen, vitamins and amino acids. Glycine is used to stimulate growth.
- Dextrose is the carbon source. Bismuth sulfite indicator is composed of bismuth ammonium citrate and sodium sulfite and acts as a selective agent for Candida species. Candida species reduce bismuth sulfite and change its color from brown to black.
- Bismuth Sulfite is also used as a selective agent against other bacteria Agar is used as the solidifying agent.
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Use: Recommended for the selective isolation, detection of Candida spp. from clinical and non-clinical samples.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Cream to light brown colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber colored opalescent solution
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pH
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6.8 ±0.2 at 25 ˚C (45.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber, opalescent gel with a slight precipitate
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