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Principle
- The media is formulated by Swan (1954) and modified by Facklam and Moody (1970). The media composition and performance criteria are as per the specification laid down in ISO 10273:1994. Bile esculin agar is composed of meat extract (Equivalent to beef extract), peptone, oxgall, ferric citrate, esculin, and agar.
- Meat extract and peptone provide nitrogen and other necessary minerals. Oxgall is a selective agent that inhibits gram-positive bacteria other than enterococci. Ferric citrate is an indicator of esculin hydrolysis.
- The esculin (glycoside) is the source of carbon. The esculin hydrolyzing microorganisms hydrolyzes esculin to esculetin and dextrose.
- The esculetin reacts with ferric citrate from a brown or black color complex. Agar is solidifying agent.
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Use: Recommended for isolating and identification of Yersinia enterocolitica from food and animal fodder.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light greenish beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light Amber colored opalescent
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pH
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6.6 ±0.2 at 25 ˚C (63.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber colored opalescent gel
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