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Principle
- Bacillus medium is used for the cultivation of Bacillus licheniformis, it is a gram-positive, spore-forming soil bacterium that is used in the biotechnology industry to manufacture enzymes, antibiotics, biochemicals, and consumer products.
- This medium is a modification of Thorne Medium (1966, 1992), recommended for the cultivation of Bacillus licheniformis. Bacillus medium contains L-glutamic acid, citric acid, Dipotassium phosphate, ferric ammonium citrate, and magnesium Sulphate.
- At the time of preparation, the media is fortified with glycerol, which provides carbon and energy for the growth of bacteria. Glutamic acid and ferric ammonium citrate act as sources of nitrogen. Citric acid provides necessary nutrients along with buffering action.
- Dipotassium phosphate acts as a buffering agent. Magnesium Sulphate is necessary for the growth of microorganisms and provides membrane stability and acts as a cofactor in various metabolic reactions.
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