Principle
- Azotobacter Mannitol agar consists of Magnesium sulfate, potassium hydrogen phosphate, Dipotassium hydrogen phosphate, calcium chloride, sodium molybdate, ferric chloride, mannitol, and agar.
- Mannitol is a carbon source. Magnesium sulfate, calcium chloride, sodium molybdate, and ferric chloride provide essential ions and sources of trace elements required for the metabolic activities of microorganisms.
- Potassium phosphates act as a buffering agent. Agar is the solidifying agent.
Use:
Recommended for the cultivation of mannitol-positive Azotobacter species from the soil.
|
PARAMETERS
|
STANDARD VALUES
|
|
PHYSICAL PARAMETERS:
|
|
Dehydrated Powder
|
Description
|
A fine, free-flowing, hygroscopic powder.
|
|
Color
|
Light beige colored powder.
|
|
Solution
|
Solubility
|
In Soluble in Distilled water at 50˚ C
|
|
Color
|
Clear slightly opalescent solution
|
|
pH
|
7.6 ±0.2 at 25 ˚C (25.40 g/ l)
|
|
Agar Plates
|
Solidification
|
40 ˚C
|
|
Color
|
Clear slightly opalescent gel
|
