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Principle
The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of a pancreatic digest of casein, papain digest of soybean, sodium chloride, dibasic potassium phosphate, dextrose, and agar. Peptone, pancreatic digestion of casein, and papain digest of soybean provide nitrogen, carbon, and essential nutrients. Sodium chloride maintains osmatic balance. Dibasic potassium phosphate acts as a buffering agent. Dextrose serves as an energy source. This medium is used as a base layer for plate assay of antibiotics.
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Use: Recommended for microbiological assay of antibiotics (Carbenicillin, colistimethate sodium, Colistin, and Polymyxin B) against a variety of microbes in accordance with United States Pharmacopoeia.
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