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Principle
The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of peptone, yeast extract, meat extract (equivalent to beef extract), dextrose, sodium chloride, and phosphates. Peptone, yeast extract, and meat peptone provide nitrogen, carbon, long-chain amino acids, vitamins, and essential nutrients for the growth of microorganisms. Dextrose serves as an energy source. Sodium chloride maintains osmotic equilibrium and phosphates act as buffering agents.
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