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Principle
The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of a pancreatic digest of casein, papaic digest of soybean, sodium chloride, dibasic potassium phosphate, dextrose, and agar. Pancreatic digestion of casein and papaic digest of soybean provide nitrogen, carbon, and other essential nutrients. Sodium chloride maintains osmatic equilibrium. Dibasic potassium phosphate resists changes in pH. Dextrose serves as an energy source. Agar is the solidifying agent. The agar concentration is reduced for the proper diffusion of the antibiotic. The Polysorbate 80 enhances the penetration of antibiotics to the cytoplasmic membrane. The AAM-10 was used as base agar for the antibiotic assay against the Pseudomonas aeruginosa and Bordetella bronchiseptia.
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