Principle
The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of peptone, yeast extract, meat extract, and agar. Peptone, yeast extract, and meat extract provide nitrogen, carbon, vitamins essential nutrients. Agar is the solidifying agent. The alkaline pH of 7.9 makes it a suitable medium for Bacillus subtilis. The AAM-5, used as seed agar as well as base agar for the Antibiotic Assay Medium against Bacillus subtilis.
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