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Principle
The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of peptone, casein peptone, yeast extract, meat extract (equivalent to beef extract), dextrose, manganese sulphate and agar. Peptone, casein peptone, yeast extract and meat peptone provide nitrogen, carbon, long-chain amino acids, vitamins and essential nutrients for the growth of microorganisms. Dextrose serves as an energy source. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis. Agar is the solidifying agent.
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