|
Principle
- Andrade peptone water is a modification of peptone water used for the biochemical identification and differentiation of different bacteria on the basis of the ability to metabolize carbohydrates and related compounds.
- This media contains an Andrade indicator, If the test organism metabolizes the added carbohydrate, acids are produced, thereby lowering the pH of the medium.
- This causes a subsequent color change of the indicator, from light pink to red. If the added carbohydrate is not metabolized, the medium remains light pink colored. Gas produced during fermentation is collected in the Durhams tube.
- The medium is dark pink when hot but becomes light pink on cooling. Test carbohydrate solutions should be sterilized separately and aseptically added to sterile Andrade peptone water.
- The biochemical identification of organisms capable of growing in this medium is made by various sugar fermentation results.
|
