Anaerobic agar is composed of tryptone, dextrose, sodium chloride, sodium thioglycolate, sodium formaldehyde sulfoxylate, methylene blue, and agar.
Tryptone acts as a source of nitrogen and dextrose is a carbon and energy source. Sodium chloride maintains osmotic equilibrium.
Sodium thioglycollate and sodium formaldehyde sulfoxylate are reducing agents. Methylene Blue serves as an indicator of anaerobiosis with a blue color indicating the presence of oxygen.
Agar is the solidifying agent.
Use: Recommended for the cultivation of anaerobic bacteria, especially Clostridium species and other anaerobic organisms from clinical and non-clinical samples
