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Principle
- Amies transport medium described by Cary and Blair (1964), is a modification of Stuart’s medium by substituting inorganic phosphates for glycerophosphate and raising the pH to 8.4.
- The inorganic phosphates make it a non-nutritive and reductive medium, which inhibits the toxic oxidative reaction and prevents self-distractions within the cell.
- The media is composed of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, monopotassium phosphate, disodium phosphate, sodium thioglycolate, and agar.
- Sodium chloride, potassium chloride, calcium chloride, and magnesium chloride serve as essential ions that help maintain osmotic balance and control the permeability of bacterial cells.
- Monopotassium phosphate and Disodium phosphate provide buffering capabilities. Sodium thioglycolate provides a reduced environment and suppresses oxidative changes. Agar is a solidifying agent.
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