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Principle
- Thioglycollate broth, the alternative is formulated as described in the N.I.H. memorandum (1955). Fluid Thioglycollate Medium is composed of tryptone, yeast extract, dextrose, sodium chloride, L-cystine, and sodium thioglycollate.
- Tryptone serves as a source of nitrogen, amino acids, and other essential nutrients. Yeast extract provides essential nutrients to the contaminants, if present.
- Dextrose serves as the energy source. Sodium chloride maintains the osmotic balance of the medium. L-cystine and sodium thioglycollate lower the oxidation-reduction potential of the medium by removing oxygen to maintain a low Eh.
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