alpha-MEM medium is modified from MEM. Compared with MEM medium, alpha-MEM is added with sodium pyruvate, zinc sulfate, vitamin B12, biotin, ascorbic acid, and other ingredients, and is widely used in the cultivation of various mammalian suspension and adherent cells. alpha-MEM is divided into two types: nucleosides and non-nucleosides.
alpha-MEM with nucleosides and deoxynucleosides is often used as a selective medium for DHFR-deficient cells.
