|
Principle
- The A-1 broth is composed of tryptone, lactose, sodium chloride, salicin, and Triton X 100.
- Tryptone serves as a source of carbon and nitrogen, vitamins and other essential nutrients required for bacterial metabolism.
- Lactose and salicin act as energy sources and sodium chloride maintains osmotic equilibrium. Triton 100 acts as a surfactant. The fecal coliforms are indicated by the lactose fermentation and gas production in the inverted Durham tube.
- The density of fecal coliform can be calculated by the standard methods using the MPN table. A-1 Medium can be used in a single-step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.
|
|
Use: Recommended for detecting fecal coliforms in water samples, wastewater, seawater, and foods by MPN Method.
|
|
PARAMETERS
|
STANDARD VALUES
|
|
PHYSICAL PARAMETERS:
|
|
Dehydrated Powder
|
Description
|
A fine, free-flowing, hygroscopic powder.
|
|
Color
|
Light beige colored powder.
|
|
Solution
|
Solubility
|
Soluble in Distilled / de-ionized water at 50˚ C
|
|
Color
|
Light amber-colored opalescent solution
|
|
pH
|
6.9±0.2 at 25˚C (31.50 g/ l)
|
|
