8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
- This assay employs the competitive inhibition enzyme immunoassay technique. The microplate provided in this kit has been pre-coated with Thromboxane B2 (TXB2) protein.
- Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thromboxane B2 (TXB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
- After the TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Thromboxane B2 (TXB2) in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit Components:
1) Pre-coated Microplate [12 strips x 8 wells]
2) 100x Biotinylated Conjugate [120ul]
3) Biotinylated Conjugate Diluent [10ml]
4) 100x Streptavidin-HRP [120ul]
5) HRP Diluent [12ml]
6) TMB Substrate Solution [9ml]
7) 25x Wash Buffer [20ml]
8) Stop reagent [6ml]
9) Lyophilized Standard [2]
10) Standard Diluent Buffer [20ml]
11) Plate Sealers [2]
12) User Manual [1]
| Size |
1 x 96 Well Plate |
| Product Category |
ELISA (RUO) |
| Reactivity |
General |
| Sample Source |
Serum; Plasma; Other Biological Fluids |
| Assay Method |
Competitive Inhibition ELISA |
| Detection Range |
93.75-6000pg/mL |
| Sensitivity |
26.29pg/mL |
| Assay Duration |
2h |
| Synonyms |
8OHdG; 7, 8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7, 8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7, 8-dihydrodeoxyguanosine |
| Storage/Stability |
-20°C/1 year; 4°C/6 months |
| Shipping |
Gel Packs |
