5-HT (5-Hydroxytryptamine) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The micro-plate provided in this kit has been pre-coated with 5-Hydroxytryptamine (5-HT) protein. Standards or samples are then added to the appropriate micro-plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine (5-HT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 5-Hydroxytryptamine (5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit Components:
1) Pre-coated Microplate [12 strips x 8 wells]
2) 100x Biotinylated Conjugate [120ul]
3) Biotinylated Conjugate Diluent [10ml]
4) 100x Streptavidin-HRP [120ul]
5) HRP Diluent [12ml]
6) TMB Substrate Solution [9ml]
7) 25x Wash Buffer [20ml]
8) Stop reagent [6ml]
9) Lyophilized Standard [2]
10) Standard Diluent Buffer [20ml]
11) Plate Sealers [2]
12) User Maual [1]