{"title":"Real Time PCR  Products","description":"","products":[{"product_id":"absolute-quantitation","title":"Absolute Quantitation","description":"\u003ch3\u003e\u003cstrong\u003eAbsolute Quantitation\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eAbsolute quantitation  assay is used to analyze the change in gene expression in a given sample relative to another reference sample (such as an untreated control sample)\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication \u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eIn various fields of science, including molecular biology, medicine, and environmental research, absolute quantitation is crucial for accurate data analysis and interpretation. Here are a few common techniques used for absolute quantitation:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eReal-time PCR (qPCR): Quantitative PCR is a widely used method to determine the absolute quantity of a specific DNA or RNA target in a sample. It relies on the amplification of the target molecule using specific primers and a fluorescent probe. By comparing the amplification curve to a standard curve generated with known concentrations of a reference sample, the absolute quantity of the target can be calculated.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDigital PCR (dPCR): Digital PCR is a technique that partitions the sample into thousands of individual reactions, each containing a limited number of target molecules or copies. By analyzing the number of positive and negative reactions, the absolute quantity of the target can be estimated. Digital PCR offers higher precision and sensitivity for absolute quantitation compared to qPCR.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMass Spectrometry: Mass spectrometry is a powerful analytical technique used for the absolute quantitation of molecules based on their mass-to-charge ratio. It can be employed to quantify proteins, peptides, metabolites, and other small molecules in a sample. Isotope-labeled internal standards or reference compounds with known concentrations are often used for accurate quantitation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eELISA (Enzyme-Linked Immunosorbent Assay): ELISA is a widely used immunoassay technique that quantifies the concentration of specific proteins or antigens in a sample. It utilizes specific antibodies that bind to the target molecule, followed by detection with an enzyme-labeled secondary antibody. By comparing the signal to a standard curve generated with known concentrations, the absolute quantity of the target can be determined.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFlow Cytometry: Flow cytometry is a technique used to quantify and analyze cells or particles in a sample. It measures the fluorescence or light scattering properties of individual cells as they pass through a laser beam. By using fluorescently labeled antibodies or specific dyes, the absolute quantity of specific cell populations or biomarkers can be determined.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThese are just a few examples of techniques used for absolute quantitation in different scientific disciplines. The choice of method depends on the nature of the molecule or substance being quantified, the available resources, and the required level of precision and sensitivity.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e GST-18%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"BioResource Biotech","offers":[{"title":"Per Expression","offer_id":42622096801981,"sku":"","price":1200.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0525\/7170\/1437\/files\/1519898853437.jpg?v=1685331638"},{"product_id":"exicycler™-v5-a-2065ruo","title":"ExiCycler™ V5, A-2065(RUO)","description":"\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cdiv class=\"line\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eInnovative Real-Time PCR System\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003enewly renovated \u003cem\u003eExiCycler\u003c\/em\u003e™ V5 Real-time PCR System maximizes your research efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1) \u003c\/strong\u003e\u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003eReduced Experiment Time\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg height=\"333\" width=\"550\" src=\"https:\/\/eng.bioneer.com\/images\/products\/Exicycler_v5_overview1.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe patented nano alloy block and temperature control technology enable ramping rates of up to 10℃\/sec, reducing PCR time to just 40 minutes.\u003c\/p\u003e\n\u003cp\u003eWe offer gradient functions, time and temperature increment options, and ramping rate control for diverse customer applications.\u003c\/p\u003e\n\u003cp\u003eUsers can select between 96-well, 96-well fast, 384-well, and 384-well fast formats, allowing selection of 4 types of thermal blocks for desired speed and number of samples.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2) Easy to Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg width=\"550\" src=\"https:\/\/eng.bioneer.com\/images\/products\/Exicycler_v5_overview3.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFor added convenience, we've included a 10-inch touchscreen and intuitive User Interface(UI).\u003c\/p\u003e\n\u003cp\u003eEasily control PCR Protocol settings, and view graphs—all without a PC.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3) True 6-color Multiplex\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg height=\"251\" width=\"500\" alt=\"\" src=\"https:\/\/eng.bioneer.com\/images\/products\/Exicycler_v5_overview4.jpg\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eExiCycler\u003c\/em\u003e™ V5 minimizes optical sensitivity and well deviations with its patented excitation light blocking technology using polarized light and\u003c\/p\u003e\n\u003cp\u003eusing polarization and simultaneous detection with a high-sensitivity CMOS camera.\u003c\/p\u003e\n\u003cp\u003eThis enables 6-channel multiplex qPCR without the need for reference dye, improving understanding of experiment reagents and reducing consumption.\u003c\/p\u003e\n\u003ch3\u003eFeatures \u0026amp; Benefits\u003c\/h3\u003e\n\u003cdiv class=\"line\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cb\u003e\u003cstrong\u003eRapid and Precise Results\u003c\/strong\u003e\u003c\/b\u003e\u003c\/span\u003e\n\u003cp\u003e\u003cspan\u003eReal-time PCR experiments now offer faster and more accurate results than ever before\u003c\/span\u003e\u003c\/p\u003e\n\u003cspan\u003e- Patented thermal block technology allows for ramping rates of up to 10.0℃\/sec\u003cbr\u003e\u003c\/span\u003e- With a running time of just 40 minutes, a multitude of experiments can be conducted within a short timeframe\u003cbr\u003e- Light Polarization Technology ensures exceptional sensitivity and accuracy\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cstrong\u003eIntuitive User Interface (UI)\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003cp\u003e\u003cspan\u003eEasy operation with a 10-inch touchscreen and user-friendly interface\u003c\/span\u003e\u003c\/p\u003e\n\u003cb\u003e\u003c\/b\u003e- Operate Independently without a PC\u003cbr\u003e- Monitor protocol and view result graphs in real time\u003cbr\u003e- Easily access past experimental files during the experiment (Open Data File Technology)\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cb\u003e6-Channel Multiplexing\u003c\/b\u003e\u003c\/span\u003e\n\u003cp\u003e\u003cspan\u003eTrue 6-channel Multiplex without the need to use reference dye\u003c\/span\u003e\u003c\/p\u003e\n- Alongside FAM, TET, TAMRA, Texas Red, and Cyanine5, the V5 model includes ATTO 425, expanding compatibility to 10 fluorescent dyes with 6 filters.\u003cbr\u003e- Wide Excitation\/Emission detection range (430nm ~ 690nm)\u003cbr\u003e- Able to test for a variety of pathogens using one sample\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cb\u003e\u003cstrong\u003eConvenience\u003c\/strong\u003e\u003c\/b\u003e\u003c\/span\u003e\n\u003cp\u003eWe assist researchers in efficiently conducting experiments\u003c\/p\u003e\n\u003cspan\u003e- Offer two types of thermal blocks that are suitable for various experimental purposes (fast\/normal)\u003cbr\u003e\u003c\/span\u003e- Both 0.1ml and 0.2ml tubes are compatible\u003cbr\u003e- Establish optimal PCR conditions using the thermal gradient function\u003cbr\u003e- Supports analysis software suitable for diverse experimental fields\u003cbr\u003e- Ensure stable performance and long lifespan by using high-performance LED\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplication\u003c\/h3\u003e\n\u003cp\u003e• Gene Expression Analysis\u003c\/p\u003e\n\u003cp\u003e• MicroRNA Expression Analysis\u003c\/p\u003e\n\u003cp\u003e• Gene Copy Number Analysis\u003c\/p\u003e\n\u003cp\u003e• Pathogen Detection\u003c\/p\u003e\n\u003cp\u003e• Genetic Mutation Testing\u003c\/p\u003e\n\u003cp\u003e• GMO Quantitative\/Qualitative Testing\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/eng.bioneer.com\/literatures\/manual\/PM-ExiCycler_V5_RUO-EN.pdf\" title=\"ExiCycler™ V5 PDF\"\u003eDownload Product Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Bioneer","offers":[{"title":"Default Title","offer_id":46132104986813,"sku":"A-2065(RUO)","price":3900000.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0525\/7170\/1437\/files\/exicycler_96_v5____c_b6a449ee-6052-4556-8f5c-00a95a05b26d.png?v=1773380305"},{"product_id":"allinonecycler™-pcr-system-a-2041","title":"AllInOneCycler™ PCR system, A-2041","description":"\u003cp\u003e\u003cem\u003eAllInOneCycler\u003c\/em\u003e\u003cspan\u003e™ is a gene amplification device that dramatically increases the ramp rate through a unique thermal block developed by Bioneer and patented special temperature control algorithm (normal: 6.5℃\/sec, fast: 9.5 ℃\/sec). In addition, 4 types of blocks are provided according to the customer's experimental field and purpose, thereby greatly improving the user's convenience.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures and Benefits\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003eShortened reaction time \u0026amp; Increased ramping rate\u003c\/b\u003e\n\u003cp\u003eIncreased ramping rate to 6.5 ° C \/ sec with the use of unique thermal block and advanced temperature control technology; up to 9.5 ° C \/ sec if equipped with special alloy thermal block * \u003cbr\u003eShortened experimental time allowing to save valuable time and energy\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_3.jpg\" width=\"556\" height=\"221\"\u003e\u003cbr\u003e\u003cb\u003eFigure1.\u003c\/b\u003e Comparison of running time and PCR results between\u003cspan\u003e AllInOneCycler\u003c\/span\u003e\u003cspan\u003e™\u003c\/span\u003e\u003csup\u003e \u003c\/sup\u003e(with normal block) and a competitor equipment\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eExcellent temperature accuracy and uniformity\u003c\/b\u003e\n\u003cp\u003eReproducible results through improved temperature uniformity and accuracy using precise temperature control system\u003c\/p\u003e\n\u003cdiv class=\"tabbody\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_5.jpg\" width=\"497\" height=\"194\"\u003e\u003cbr\u003e\u003cb\u003eFigure 2.\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eComparison of PCR results in 24 wells of 96-well block and 384-well block\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eInterchangeable thermal block\u003c\/b\u003e\n\u003cp\u003eSelective use with interchangeable thermal blocks capable of choosing between 4 of those types depending on the purpose of the experiment\u003cbr\u003e96-well, 384-wells included for comprehensive choice of sample sizes as well as normal and special alloy thermal blocks for required ramp rate \u003c\/p\u003e\n\u003cdiv class=\"tabbody\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_Thermalblock_Fig4.jpg\" width=\"200\" height=\"165\"\u003e     \u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_Thermalblock_Fig3.png\" width=\"500\" height=\"145\"\u003e\u003cbr\u003e\u003cb\u003eFigure 3.\u003c\/b\u003e Diagram showing two types of thermal blocks along with the method to replace them (left: 96-well, right: 384-well)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eThermal gradient function\u003c\/b\u003e\n\u003cp\u003eMinimized time and reduced experimental protocols to easily find the optimum test conditions at once just by using the thermal gradient function\u003c\/p\u003e\n\u003cdiv class=\"tabbody\"\u003e\n\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_8.jpg\" width=\"602\" height=\"192\"\u003e\u003cbr\u003e\n\u003cp\u003e\u003cb\u003eFigure 4.\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003eSetting the PCR condition using the thermal gradient PCR function in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eAllInOneCycleler\u003c\/em\u003e™ (human genomic DNA, CFS2 gene, 830 bp)\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eEasy to use interface\u003c\/b\u003e\n\u003cp\u003e\u003cbr\u003eHigh convenience through 7-inch LCD touch screen and user-friendly UI with 8 template protocols provided for a simple set up\u003cbr\u003eEasily control multiple instruments simultaneously and monitor their massive sample sizes by using a PC program provided separately from us\u003c\/p\u003e\n\u003cdiv class=\"tabbody\"\u003e\n\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_9.jpg\" width=\"573\" height=\"135\"\u003e\u003cbr\u003e\n\u003cp\u003e\u003cb\u003eFigure 5.\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eScreen composition of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eAllInOneCycler\u003c\/em\u003e™. (a) Start screen, (b) Protocol creation and modification screen, (c) Template protocol selection screen\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eCompact and simple design\u003c\/b\u003e\n\u003cp\u003eSimple, sophisticated design with a small size making it easy to be moved around and placed even in a small laboratory with a narrow space\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/eng.bioneer.com\/images\/products\/AllInOneCycler_Figure6.png\" width=\"847\" height=\"auto\"\u003e\u003c\/p\u003e\n\u003cbr\u003e\u003cb\u003eFigure 6.\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eFront, back, side design of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eAllInOneCycler\u003c\/em\u003e™\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Bioneer","offers":[{"title":"Default Title","offer_id":46208771358909,"sku":"A-2041","price":0.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0525\/7170\/1437\/files\/allinonecycler.jpg?v=1773380377"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0525\/7170\/1437\/collections\/Real-Time-PCR-Principle-Process-Markers-Advantages-Uses.webp?v=1670993006","url":"https:\/\/ebiostore.com\/collections\/real-time-pcr-products.oembed","provider":"eBiostore.com","version":"1.0","type":"link"}