Principle
- Murashige & Skoog medium has been developed for in vitro use for micropropagation, organ culture, callus culture, and suspension culture.
- The formulation comprises macronutrients, micronutrients, carbon sources, amino acids, and vitamins. The macronutrients contain essential elements like nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S) for satisfactory growth and morphogenesis.
- The micronutrients include iron (Fe), manganese (Mn), zinc (Zn), boron (B), copper (Cu), and molybdenum (Mo) required for the growth of plant tissue and cells.
- The media can be agglutinated with potassium iodide, vitamins, sucrose, and agar or phyta gel.
- Amino acids are used for optimal growth, they are essential for establishing cultures of cells and protoplasts. Amino acids provide plant cells with a source of nitrogen that is easily assimilated by tissues and cells faster than inorganic nitrogen sources.
Use
Recommendations for micropropagation, organ culture, callus culture, and suspension culture.
