fT4 (Free Thyroxine) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The micro-plate provided in this kit has been pre-coated with Free Thyroxine (fT4) protein. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Free Thyroxine (fT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Free Thyroxine (fT4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit Components:
1) Pre-coated Microplate [12 strips x 8 wells]
2) 100x Biotinylated Conjugate [120ul]
3) Biotinylated Conjugate Diluent [10ml]
4) 100x Streptavidin-HRP [120ul]
5) HRP Diluent [12ml]
6) TMB Substrate Solution [9ml]
7) 25x Wash Buffer [20ml]
8) Stop reagent [6ml]
9) Lyophilized Standard [2]
10) Standard Diluent Buffer [20ml]
11) Plate Sealers [2]
12) User Manual [1]