Principle
- These mediums contain malic acid which serves as the prime carbon source and favors the growth of Azospirillum and prevents the overgrowth of other nitrogen fixers.
- Dipotassium phosphates help to maintain the pH of the medium. Necessary trace elements like ferrous Sulphate, manganese Sulphate, and sodium molybdate support the growth of the Azospirillum species.
- Divalentsalts like calcium and magnesium help the metabolism of cells. Sodium chloride helps to maintain the osmotic balance of cells.
- Due to the nitrogen fixation by diazotroph bacteria ammonia is produced, and the color of the medium changes from light yellow color to greenish blue or blue (Bromothymol blue pH indicator).
Use:
Recommended for the cultivation of Azospirillum species.
|
PARAMETERS
|
STANDARD VALUES
|
|
PHYSICAL PARAMETERS:
|
|
Dehydrated Powder
|
Description
|
A fine, free-flowing, hygroscopic powder.
|
|
Color
|
Part A: Cream to yellow homogeneous free-flowing powder Part B: White to cream pellets.
|
|
Solution
|
Solubility
|
Soluble in Distilled / de-ionized water at 50˚ C
|
|
Color
|
Light yellow to pale green colored solution
|
|
pH
|
6.8 ±0.2 at 25 ˚C (8.10g part A and 4g part B)
|
