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Principle
- Asparagine proline broth is an enrichment broth for Pseudomonas aeruginosa. The composition is strictly mineral base with enantiomeric forms of asparagine and proline, magnesium sulfate, dipotassium sulfate, and potassium sulfate.
- DL-asparagine and proline serve as the sole source of nitrogen. The dipotassium and potassium salts act as a buffer system and magnesium sulfate performs multiple functions, magnesium ion is required in a large variety of enzymatic reactions, and reproduction and also acts as a buffer.
- Pseudomonas aeruginosa hydrolyze asparagine to aspartic acid and reproduce vigorously, the appearance of growth with or without fluorescent pigmentation is considered a presumptive test for P. aeruginosa.
- The microbial count can be determined by using the MPN test and conformation need to be done by subculturing on cetrimide agar.
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